Part:BBa_K2548013:Design
LacI - pTac - Scygonadin + 6xHis Tag - Terminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1442
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Scygonadin is an anionic antimicrobial peptide identified from the seminal plasma of the species Scylla serrata (Giant mud crab). It has shown the antimicrobial activity against A. hydrophila. Its DNA reveals that it is only expressed in male crabs, helping them to fight off bacterial invasions. However, it is difficult to transfer a large amount of scygonadin from Scylla serrata to other organisms, so our plan is to produce peptide syngonadin in E. coli through genetic modification. Our aim is to insert the cDNA sequence coding for mature peptides of scygonadin into the genetic expression of E. coli.
Source
Huang, W. S., Wang, K. J., Yang, M., Cai, J. J., Li, S. J., & Wang, G. Z. (2006). Purification and part characterization of a novel antibacterial protein Scygonadin, isolated from the seminal plasma of mud crab, Scylla serrata (Forskål, 1775). Journal of Experimental Marine Biology and Ecology, 339(1), 37-42. doi:10.1016/j.jembe.2006.06.029